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PIP4K2C ELISA Kit (Bovine)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Phosphatidylinositol-5-phosphate 4-kinase type 2 gamma

Gene Aliases

Phosphatidylinositol 5-phosphate 4-kinase type II gamma; phosphatidylinositol 5-phosphate 4-kinase type-2 gamma; PI (5) P 4-kinase type II gamma; PIP4KII-gamma.

Gene ID

540005

Accession Number

NP_001069664

Reactivity

Bos taurus, Bovine

Target

Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pip4k2c ELISA Kit (Bovine) (OKEH06683) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pip4k2c has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pip4k2c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pip4k2c captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.156-10ng/ml

Sensitivity

0.069 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma

NCBI Gene Symbol

PIP4K2C

Protein Name

Q0P5F7

Gene Name URL

PIP4K2C

Nucleotide Accession Number

NM_001076196

Frequently Asked Questions

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