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NT5C3B ELISA Kit (Chicken)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

5'-nucleotidase, cytosolic IIIB

Gene Aliases

5'-nucleotidase, cytosolic III-like;7-methylguanosine nucleotidase;7-methylguanosine phosphate-specific 5'-nucleotidase; cN-III-like protein; cytosolic 5'-nucleotidase 3B; cytosolic 5'-nucleotidase III-like protein; N (7) -methylguanylate 5'-phosphatase; NT5C3L.

Gene ID

420034

Accession Number

NP_001006334

Reactivity

Chicken, Gallus gallus

Target

7-methylguanosine phosphate-specific 5'-nucleotidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Nt5c3b ELISA Kit (Chicken) (OKEH06654) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nt5c3b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nt5c3b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nt5c3b captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.78-50ng/ml

Sensitivity

0.29 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken Cytosolic 5'-nucleotidase III-like protein

NCBI Gene Symbol

NT5C3B

Protein Name

Q5ZKF6

Gene Name URL

NT5C3B

Nucleotide Accession Number

NM_001006334

Frequently Asked Questions

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