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SH3GL1 ELISA Kit (Bovine)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

SH3 domain containing GRB2 like 1, endophilin A2

Gene Aliases

Endophilin-2; endophilin-A2; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1; SH3-domain GRB2-like 1.

Gene ID

512719

Accession Number

NP_001039595

Reactivity

Bos taurus, Bovine

Target

Endophilin-A2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Sh3gl1 ELISA Kit (Bovine) (OKEH06644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sh3gl1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sh3gl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Sh3gl1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.78-50ng/ml

Sensitivity

0.29 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine Endophilin-A2

NCBI Gene Symbol

SH3GL1

Protein Name

Q2KJA1

Gene Name URL

SH3GL1

Nucleotide Accession Number

NM_001046130

Frequently Asked Questions

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