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HRAS ELISA Kit (Chicken)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

HRas proto-oncogene, GTPase

Gene Aliases

C-H-ras; GTPase HRas; Harvey rat sarcoma viral oncogene homolog; H-RAS; H-Ras-1; HRAS1; p21ras; transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ha-ras Harvey rat sarcoma viral oncogene-like protein.

Gene ID

396229

Accession Number

NP_990623

Reactivity

Chicken, Gallus gallus

Target

GTPase HRas

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hras ELISA Kit (Chicken) (OKEH06637) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hras has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hras is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hras captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.156-10ng/ml

Sensitivity

0.057 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Chicken GTPase HRas

NCBI Gene Symbol

HRAS

Protein Name

P08642

Gene Name URL

HRAS

Nucleotide Accession Number

NM_205292

Frequently Asked Questions

More Discoveries

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