HRAS ELISA Kit (Chicken)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
HRas proto-oncogene, GTPase
Gene Aliases
C-H-ras; GTPase HRas; Harvey rat sarcoma viral oncogene homolog; H-RAS; H-Ras-1; HRAS1; p21ras; transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ha-ras Harvey rat sarcoma viral oncogene-like protein.
Gene ID
396229
Accession Number
NP_990623
Reactivity
Chicken, Gallus gallus
Target
GTPase HRas
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hras ELISA Kit (Chicken) (OKEH06637) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hras has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hras is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hras captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/ml
Sensitivity
0.057 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Chicken GTPase HRas
NCBI Gene Symbol
HRAS
Protein Name
P08642
Gene Name URL
HRAS
Nucleotide Accession Number
NM_205292
Frequently Asked Questions
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