HSPA6 ELISA Kit (Pig)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Heat shock protein family A (Hsp70) member 6
Gene Aliases
Heat shock 70 kDa protein; Heat shock 70 kDa protein 6; heat shock 70 kDa protein B'; heat shock 70kDa protein 6 (HSP70B') ; heat shock protein 70; HSP70; HSP70B'.
Gene ID
396906
Accession Number
NP_001116599
Reactivity
Porcine, Sus scrofa
Target
Heat shock 70 kDa protein 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hspa6 ELISA Kit (Pig) (OKEH06624) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspa6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspa6 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
31.2-2000pg/ml
Sensitivity
7.7 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Pig Heat shock 70 kDa protein 6
NCBI Gene Symbol
HSPA6
Protein Name
Q04967
Gene Name URL
HSPA6
Nucleotide Accession Number
NM_001123127
Frequently Asked Questions
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