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HSPA6 ELISA Kit (Pig)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Heat shock protein family A (Hsp70) member 6

Gene Aliases

Heat shock 70 kDa protein; Heat shock 70 kDa protein 6; heat shock 70 kDa protein B'; heat shock 70kDa protein 6 (HSP70B') ; heat shock protein 70; HSP70; HSP70B'.

Gene ID

396906

Accession Number

NP_001116599

Reactivity

Porcine, Sus scrofa

Target

Heat shock 70 kDa protein 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Hspa6 ELISA Kit (Pig) (OKEH06624) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspa6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspa6 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

31.2-2000pg/ml

Sensitivity

7.7 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Pig Heat shock 70 kDa protein 6

NCBI Gene Symbol

HSPA6

Protein Name

Q04967

Gene Name URL

HSPA6

Nucleotide Accession Number

NM_001123127

Frequently Asked Questions

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