What is the catalog number for PCK1 ELISA Kit (Bovine)?

The catalog number for PCK1 ELISA Kit (Bovine) is OKEH06610-96W.

What sizes are available for PCK1 ELISA Kit (Bovine)?

PCK1 ELISA Kit (Bovine) is available in the following size(s): 96 Well.

Is PCK1 ELISA Kit (Bovine) in stock?

PCK1 ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

PCK1 ELISA Kit (Bovine)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Phosphoenolpyruvate carboxykinase 1

Gene Aliases

PEPCK-C; phosphoenolpyruvate carboxykinase 1 (soluble) ; phosphoenolpyruvate carboxykinase 1 variant 1; phosphoenolpyruvate carboxykinase 1 variant 2; phosphoenolpyruvate carboxykinase 1 variant 3; phosphoenolpyruvate carboxykinase 1 variant 4; phosphoenolpyruvate carboxykinase 1 variant 5; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; PPCK1; serine-protein kinase PCK1.

Gene ID

282855

Accession Number

NP_777162

Reactivity

Bos taurus, Bovine

Target

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pck1 ELISA Kit (Bovine) (OKEH06610) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pck1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pck1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pck1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol