LIPC ELISA Kit (Bovine)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Lipase C, hepatic type
Gene Aliases
Hepatic lipase; hepatic triacylglycerol lipase; HL; lipase member C; lipase, hepatic; lysophospholipase; phospholipase A1.
Gene ID
535192
Accession Number
NP_001030487
Reactivity
Bos taurus, Bovine
Target
Hepatic triacylglycerol lipase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lipc ELISA Kit (Bovine) (OKEH06602) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lipc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lipc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lipc captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
7.8-500ng/ml
Sensitivity
3.6 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Bovine Hepatic triacylglycerol lipase
NCBI Gene Symbol
LIPC
Protein Name
Q3SZ79
Gene Name URL
LIPC
Nucleotide Accession Number
NM_001035410
Frequently Asked Questions
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