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GABRA2 ELISA Kit (Bovine)

Product Specifications

Reproducibility

Inter-Assay CV

CAS Number

7732-18-5

Gene Name

Gamma-aminobutyric acid type A receptor alpha2 subunit, gamma-aminobutyric acid type A receptor subunit alpha2

Gene Aliases

GABA (A) receptor subunit alpha-2; gamma-aminobutyric acid (GABA) A receptor, alpha 2; gamma-aminobutyric acid receptor subunit alpha-2; gamma-aminobutyric acid type A receptor alpha2 subunit.

Gene ID

104972746, 282236

Accession Number

NP_776966

Reactivity

Bos taurus, Bovine

Target

Gamma-aminobutyric acid receptor subunit alpha-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Gabra2 ELISA Kit (Bovine) (OKEH06590) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gabra2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gabra2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gabra2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

78-5000pg/ml

Sensitivity

19 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Bovine Gamma-aminobutyric acid receptor subunit alpha-2

NCBI Gene Symbol

GABRA2

Protein Name

P10063

Gene Name URL

GABRA2

Nucleotide Accession Number

NM_174541

Frequently Asked Questions

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