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STK17B ELISA Kit (Human)

Selected Variant

OKEH06559-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Serine/threonine kinase 17b

Gene Aliases

DAP kinase-related apoptosis-inducing protein kinase 2; death-associated protein kinase-related 2; DRAK2; serine/threonine-protein kinase 17B.

Gene ID

9262

Accession Number

NP_008822

Reactivity

Homo sapiens, Human

Target

Serine/threonine-protein kinase 17B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology STK17B ELISA Kit (Human) (OKEH06559) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for STK17B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for STK17B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample STK17B captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 103.6%

Sensitivity

0.087 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Serine/threonine-protein kinase 17B

NCBI Gene Symbol

STK17B

Protein Name

O94768

Gene Name URL

STK17B

Nucleotide Accession Number

NM_006891

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