FAM210A ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Family with sequence similarity 210 member A
Gene Aliases
C18orf19; HsT2329; protein FAM210A; uncharacterized protein C18orf19.
Gene ID
125228
Accession Number
NP_001257695
Reactivity
Homo sapiens, Human
Target
Protein FAM210A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology FAM210A ELISA Kit (Human) (OKEH06558) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-FAM210A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FAM210A and incubated. The FAM210A found in the sample or standards competes with the biotinylated FAM210A for limited binding sites on the immobilized anti-FAM210A antibody. Excess unbound biotinylated FAM210A and sample or standard FAM210A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FAM210A captured in the well and inversely proportional to the amount of FAM210A which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 105%
Sensitivity
0.184 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Protein FAM210A
NCBI Gene Symbol
FAM210A
Protein Name
Q96ND0
Gene Name URL
FAM210A
Nucleotide Accession Number
NM_001270766
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