ZAK ELISA Kit (Human)

AZK; CNM6; HCCS-4; human cervical cancer suppressor gene 4 protein; Leucine zipper- and sterile alpha motif-containing kinase; mitogen-activated protein kinase kinase kinase 20; mitogen-activated protein kinase kinase kinase MLT; mixed lineage kinase 7; mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; mixed lineage kinase-related kinase MRK-beta; MLK7; mlklak; MLK-like mitogen-activated protein triple kinase; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; pk; SFMMP; sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; ZAK; ZAK1 homolog, leucine zipper and sterile-alpha motif kinase.

Aviva Systems Biology ZAK ELISA Kit (Human) (OKEH06551) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-ZAK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZAK and incubated. The ZAK found in the sample or standards competes with the biotinylated ZAK for limited binding sites on the immobilized anti-ZAK antibody. Excess unbound biotinylated ZAK and sample or standard ZAK is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZAK captured in the well and inversely proportional to the amount of ZAK which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol