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SET ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

SET nuclear proto-oncogene

Gene Aliases

2PP2A; chromatin remodelling factor; HLA-DR-associated protein II; I2PP2A; IGAAD; inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; IPP2A2; MRD58; PHAPII; phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor; protein SET; SET nuclear oncogene; SET translocation (myeloid leukemia-associated) ; TAF-I; TAF-IBETA; Template-activating factor I; Template-Activating Factor-I, chromatin remodelling factor.

Gene ID

6418

Accession Number

NP_001146918

Reactivity

Homo sapiens, Human

Target

Protein SET

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SET ELISA Kit (Human) (OKEH06547) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SET has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SET is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SET captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 88%

Sensitivity

0.176 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Protein SET

NCBI Gene Symbol

SET

Protein Name

Q01105

Gene Name URL

SET

Nucleotide Accession Number

NM_001153446

Frequently Asked Questions

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