SET ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
SET nuclear proto-oncogene
Gene Aliases
2PP2A; chromatin remodelling factor; HLA-DR-associated protein II; I2PP2A; IGAAD; inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; IPP2A2; MRD58; PHAPII; phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor; protein SET; SET nuclear oncogene; SET translocation (myeloid leukemia-associated) ; TAF-I; TAF-IBETA; Template-activating factor I; Template-Activating Factor-I, chromatin remodelling factor.
Gene ID
6418
Accession Number
NP_001146918
Reactivity
Homo sapiens, Human
Target
Protein SET
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SET ELISA Kit (Human) (OKEH06547) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SET has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SET is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SET captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 88%
Sensitivity
0.176 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Protein SET
NCBI Gene Symbol
SET
Protein Name
Q01105
Gene Name URL
SET
Nucleotide Accession Number
NM_001153446
Frequently Asked Questions
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