What is the catalog number for GCDH ELISA Kit (Human)?

The catalog number for GCDH ELISA Kit (Human) is OKEH06540-96W.

What sizes are available for GCDH ELISA Kit (Human)?

GCDH ELISA Kit (Human) is available in the following size(s): 96 Well.

Is GCDH ELISA Kit (Human) in stock?

GCDH ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

GCDH ELISA Kit (Human)

Selected Variant

OKEH06540-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Glutaryl-CoA dehydrogenase

Gene Aliases

ACAD5; GCD; glutaryl-CoA dehydrogenase, mitochondrial; glutaryl-Coenzyme A dehydrogenase; truncated GCDH; truncated glutaryl-CoA dehydrogenase.

Gene ID

2639

Accession Number

NP_001188299

Reactivity

Homo sapiens, Human

Target

Glutaryl-CoA dehydrogenase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GCDH ELISA Kit (Human) (OKEH06540) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GCDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GCDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GCDH captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 103%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Glutaryl-CoA dehydrogenase, mitochondrial

NCBI Gene Symbol

GCDH

Protein Name

Q92947

Gene Name URL

GCDH

Nucleotide Accession Number

NM_001201370

Price

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

CD44 Standard (156-3C11), CF647 conjugate, 0.1mg/mL

BNC470460-100 1x 100 µL