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HIF3A ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Hypoxia inducible factor 3 subunit alpha

Gene Aliases

Basic-helix-loop-helix-PAS protein MOP7; bHLHe17; class E basic helix-loop-helix protein 17; HIF-3A; HIF3-alpha-1; hypoxia inducible factor 3 alpha subunit; hypoxia-inducible factor 3-alpha; inhibitory PAS domain protein; IPAS; member of PAS protein 7; MOP7; PAS domain-containing protein 7; PASD7.

Gene ID

64344

Accession Number

NP_001078518

Reactivity

Homo sapiens, Human

Target

Hypoxia-inducible factor 3-alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HIF3A ELISA Kit (Human) (OKEH06537) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIF3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIF3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIF3A captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 96%

Sensitivity

39.1 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Hypoxia-inducible factor 3-alpha

NCBI Gene Symbol

HIF3A

Protein Name

Q9Y2N7

Gene Name URL

HIF3A

Nucleotide Accession Number

NM_001085049

Frequently Asked Questions

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