HIF3A ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Hypoxia inducible factor 3 subunit alpha
Gene Aliases
Basic-helix-loop-helix-PAS protein MOP7; bHLHe17; class E basic helix-loop-helix protein 17; HIF-3A; HIF3-alpha-1; hypoxia inducible factor 3 alpha subunit; hypoxia-inducible factor 3-alpha; inhibitory PAS domain protein; IPAS; member of PAS protein 7; MOP7; PAS domain-containing protein 7; PASD7.
Gene ID
64344
Accession Number
NP_001078518
Reactivity
Homo sapiens, Human
Target
Hypoxia-inducible factor 3-alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HIF3A ELISA Kit (Human) (OKEH06537) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIF3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIF3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIF3A captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 96%
Sensitivity
39.1 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Hypoxia-inducible factor 3-alpha
NCBI Gene Symbol
HIF3A
Protein Name
Q9Y2N7
Gene Name URL
HIF3A
Nucleotide Accession Number
NM_001085049
Frequently Asked Questions
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