TYRP1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Tyrosinase related protein 1
Gene Aliases
5,6-dihydroxyindole-2-carboxylic acid oxidase; b-PROTEIN; CAS2; catalase B; CATB; DHICA oxidase; glycoprotein 75; GP75; melanoma antigen gp75; OCA3; TRP; TRP1; Tyrosinase-related protein 1; TYRP.
Gene ID
7306
Accession Number
NP_001975
Reactivity
Homo sapiens, Human
Target
5,6-dihydroxyindole-2-carboxylic acid oxidase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TYRP1 ELISA Kit (Human) (OKEH06533) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TYRP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TYRP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TYRP1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88%
Sensitivity
0.418 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human 5,6-dihydroxyindole-2-carboxylic acid oxidase
NCBI Gene Symbol
TYRP1
Protein Name
P17643
Gene Name URL
TYRP1
Nucleotide Accession Number
NM_001984
Frequently Asked Questions
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