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MZB1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Marginal zone B and B1 cell specific protein

Gene Aliases

Caspase-2 binding protein; HSPC190; marginal zone B- and B1-cell-specific protein; MEDA-7; mesenteric estrogen-dependent adipose 7; mesenteric oestrogen-dependent adipose gene- 7; PACAP; pERp1; plasma cell-induced ER protein 1; plasma cell-induced resident endoplasmic reticulum protein; plasma cell-induced resident ER protein; proapoptotic caspase adapter protein; proapoptotic caspase adaptor protein.

Gene ID

51237

Accession Number

NP_071907

Reactivity

Homo sapiens, Human

Target

Marginal zone B- and B1-cell-specific protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MZB1 ELISA Kit (Human) (OKEH06532) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MZB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MZB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MZB1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 99%

Sensitivity

39.6 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Plasma cell-induced resident endoplasmic reticulum protein

NCBI Gene Symbol

MZB1

Protein Name

Q8WU39

Gene Name URL

MZB1

Nucleotide Accession Number

NM_022462

Frequently Asked Questions

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