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HNF1B ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

HNF1 homeobox B

Gene Aliases

ADTKD3; FJHN; hepatocyte nuclear factor 1-beta; HNF1 beta A; HNF-1B; HNF1beta; HNF-1-beta; HNF2; homeoprotein LFB3; HPC11; LFB3; LF-B3; MODY5; RCAD; T2D; TCF2; TCF-2; Transcription factor 2; transcription factor 2, hepatic; Variant hepatic nuclear factor 1; VHNF1.

Gene ID

6928

Accession Number

NP_001310298

Reactivity

Homo sapiens, Human

Target

Hepatocyte nuclear factor 1-beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HNF1B ELISA Kit (Human) (OKEH06455) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HNF1B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HNF1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HNF1B captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 88%

Sensitivity

0.091 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human Hepatocyte nuclear factor 1-beta

NCBI Gene Symbol

HNF1B

Protein Name

P35680

Gene Name URL

HNF1B

Nucleotide Accession Number

NM_001323369

Frequently Asked Questions

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