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FGF8 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Fibroblast growth factor 8

Gene Aliases

Ai; Aigf; androgen-induced growth factor; Fgf6c; Fgf-8; fibroblast growth factor 6c; fibroblast growth factor 8; HBGF-8; heparin-binding growth factor 8.

Gene ID

14179

Accession Number

NP_033775

Reactivity

Mouse, Mus musculus

Target

Fibroblast growth factor 8

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Fgf8 ELISA Kit (Mouse) (OKEH06454) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgf8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fgf8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgf8 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 91.2%

Sensitivity

15.73 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Fibroblast growth factor 8

NCBI Gene Symbol

Fgf8

Protein Name

P37237

Gene Name URL

Fgf8

Nucleotide Accession Number

NM_009645

Frequently Asked Questions

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