ERCC5 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
ERCC excision repair 5, endonuclease
Gene Aliases
COFS3; DNA excision repair protein ERCC-5; DNA repair protein complementing XP-G cells; ERCC5-201; ERCM2; excision repair cross-complementation group 5; excision repair cross-complementing rodent repair deficiency, complementation group 5; UVDR; Xeroderma pigmentosum group G-complementing protein; xeroderma pigmentosum, complementation group G; XPG; XPGC; XPG-complementing protein.
Gene ID
2073
Accession Number
NP_038642
Reactivity
Homo sapiens, Human
Target
DNA repair protein complementing XP-G cells
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ERCC5 ELISA Kit (Human) (OKEH06403) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERCC5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ERCC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERCC5 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20U/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 98%
Sensitivity
0.156U/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human DNA repair protein complementing XP-G cells
NCBI Gene Symbol
ERCC5
Protein Name
P28715
Gene Name URL
ERCC5
CAS Number
7732-18-5
Nucleotide Accession Number
NM_013614
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