What is the catalog number for APAF1 ELISA Kit (Mouse)?

The catalog number for APAF1 ELISA Kit (Mouse) is OKEH06399-96W.

What sizes are available for APAF1 ELISA Kit (Mouse)?

APAF1 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is APAF1 ELISA Kit (Mouse) in stock?

APAF1 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

APAF1 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Apoptotic peptidase activating factor 1

Gene Aliases

6230400I06Rik; Ap; Apaf-1; Apaf1l; apoptotic protease-activating factor 1; fog; forebrain overgrowth; mKIAA0413.

Gene ID

11783

Accession Number

NP_032946

Reactivity

Mouse, Mus musculus

Target

Apoptotic protease-activating factor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Apaf1 ELISA Kit (Mouse) (OKEH06399) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apaf1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apaf1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apaf1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol