What is the catalog number for NPHS1 ELISA Kit (Mouse)?

The catalog number for NPHS1 ELISA Kit (Mouse) is OKEH06396-96W.

What sizes are available for NPHS1 ELISA Kit (Mouse)?

NPHS1 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is NPHS1 ELISA Kit (Mouse) in stock?

NPHS1 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

NPHS1 ELISA Kit (Mouse)

Selected Variant

OKEH06396-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Nephrosis 1, nephrin

Gene Aliases

Ne; nephrin; nephrin 1; NephrinB; nephrosis 1 homolog, nephrin; renal glomerulus-specific cell adhesion receptor.

Gene ID

54631

Accession Number

NP_001292988

Reactivity

Mouse, Mus musculus

Target

Nephrin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Nphs1 ELISA Kit (Mouse) (OKEH06396) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nphs1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nphs1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nphs1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 97.6%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Nephrin

NCBI Gene Symbol

Nphs1

Protein Name

Q9QZS7

Gene Name URL

Nphs1

Nucleotide Accession Number

NM_001306059

Price

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