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CARTPT ELISA Kit (Mouse)

Product Specifications

Reproducibility

_x005F Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

CART prepropeptide

Gene Aliases

Ca; Cart; cocaine- and amphetamine-regulated transcript protein.

Gene ID

27220

Accession Number

NP_072112

Reactivity

Mouse, Mus musculus

Target

Cocaine- and amphetamine-regulated transcript protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Cartpt ELISA Kit (Mouse) (OKEH06377) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cartpt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cartpt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cartpt captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 90%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Cocaine- and amphetamine-regulated transcript protein

NCBI Gene Symbol

Cartpt

Protein Name

P56388

Gene Name URL

Cartpt

Nucleotide Accession Number

NM_022590

Frequently Asked Questions

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