PDGFC ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Platelet-derived growth factor, C polypeptide
Gene Aliases
1110064L01Rik; AI647969; fallotein; PDGF-; PDGF-C; platelet-derived growth factor C; SCDGF; spinal cord-derived growth factor; VEGF-E.
Gene ID
54635
Accession Number
NP_076463
Reactivity
Mouse, Mus musculus
Target
Platelet-derived growth factor C
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pdgfc ELISA Kit (Mouse) (OKEH06376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdgfc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pdgfc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdgfc captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100ng/mL
Sensitivity
0.85 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Platelet-derived growth factor C
NCBI Gene Symbol
Pdgfc
Protein Name
Q8CI19
Gene Name URL
Pdgfc
Nucleotide Accession Number
NM_023973
Frequently Asked Questions
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