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PDGFC ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Platelet-derived growth factor, C polypeptide

Gene Aliases

1110064L01Rik; AI647969; fallotein; PDGF-; PDGF-C; platelet-derived growth factor C; SCDGF; spinal cord-derived growth factor; VEGF-E.

Gene ID

54635

Accession Number

NP_076463

Reactivity

Mouse, Mus musculus

Target

Platelet-derived growth factor C

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pdgfc ELISA Kit (Mouse) (OKEH06376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdgfc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pdgfc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdgfc captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100ng/mL

Sensitivity

0.85 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Platelet-derived growth factor C

NCBI Gene Symbol

Pdgfc

Protein Name

Q8CI19

Gene Name URL

Pdgfc

Nucleotide Accession Number

NM_023973

Frequently Asked Questions

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