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VEGFD ELISA Kit (Mouse)

Selected Variant

OKEH06361-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Vascular endothelial growth factor D

Gene Aliases

AI325264; c-fos induced growth factor; c-Fos-induced growth factor; Fig; Figf; vascular endothelial growth factor D; VEGF-; VEGF-D.

Gene ID

14205

Accession Number

NP_032999

Reactivity

Mouse, Mus musculus

Target

Vascular endothelial growth factor D

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Vegfd ELISA Kit (Mouse) (OKEH06361) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vegfd has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Vegfd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Vegfd captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 82.3%

Sensitivity

7.92 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse Vascular endothelial growth factor D

NCBI Gene Symbol

Vegfd

Protein Name

P97946

Gene Name URL

Vegfd

Nucleotide Accession Number

NM_008973

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