RPS14 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Ribosomal protein S14
Gene Aliases
40S ribosomal protein S14; emetine resistance; EMTB; S14; small ribosomal subunit protein uS11.
Gene ID
6208
Accession Number
NP_839972
Reactivity
Homo sapiens, Human
Target
40S ribosomal protein S14
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RPS14 ELISA Kit (Human) (OKEH06343) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-RPS14 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RPS14 and incubated. The RPS14 found in the sample or standards competes with the biotinylated RPS14 for limited binding sites on the immobilized anti-RPS14 antibody. Excess unbound biotinylated RPS14 and sample or standard RPS14 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RPS14 captured in the well and inversely proportional to the amount of RPS14 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 94%
Sensitivity
0.175 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human 40S ribosomal protein S14
NCBI Gene Symbol
RPS14
Protein Name
P62263
Gene Name URL
RPS14
Nucleotide Accession Number
NM_178241
Frequently Asked Questions
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