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RPS14 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Ribosomal protein S14

Gene Aliases

40S ribosomal protein S14; emetine resistance; EMTB; S14; small ribosomal subunit protein uS11.

Gene ID

6208

Accession Number

NP_839972

Reactivity

Homo sapiens, Human

Target

40S ribosomal protein S14

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RPS14 ELISA Kit (Human) (OKEH06343) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-RPS14 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RPS14 and incubated. The RPS14 found in the sample or standards competes with the biotinylated RPS14 for limited binding sites on the immobilized anti-RPS14 antibody. Excess unbound biotinylated RPS14 and sample or standard RPS14 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RPS14 captured in the well and inversely proportional to the amount of RPS14 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 94%

Sensitivity

0.175 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Human 40S ribosomal protein S14

NCBI Gene Symbol

RPS14

Protein Name

P62263

Gene Name URL

RPS14

Nucleotide Accession Number

NM_178241

Frequently Asked Questions

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