CADM3 ELISA Kit (Mouse)

BI; BIgR; cell adhesion molecule 3; Igsf; Igsf4b; Immunoglobulin superfamily member 4B; immunoglobulin superfamily, member 4B; Nec; Necl; Necl1; Necl-1; nectin-like protein 1; nectin-lke 1; synaptic cell adhesion molecule 3; SynC; SynCAM3; Ts; TSLC1-like protein 1; Tsll1.

Aviva Systems Biology Cadm3 ELISA Kit (Mouse) (OKEH06340) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Cadm3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Cadm3 and incubated. The Cadm3 found in the sample or standards competes with the biotinylated Cadm3 for limited binding sites on the immobilized anti-Cadm3 antibody. Excess unbound biotinylated Cadm3 and sample or standard Cadm3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Cadm3 captured in the well and inversely proportional to the amount of Cadm3 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol