SERPINH1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Serpin family H member 1
Gene Aliases
47 kDa heat shock protein; Cbp2; collagen binding protein 2; collagen-binding protein; colligin; GP46; serine (or cysteine) peptidase inhibitor, clade H, member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2; serine (or cysteine) proteinase inhibitor, clade H, member 1; serine proteinase inhibitor, clade H (heat shock protein 47), member 1; serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) ; serpin peptidase inhibitor, clade H, member 1; Serpinh2.
Gene ID
29345
Accession Number
NP_058869.1
Reactivity
Rat, Rattus norvegicus
Target
Serpin H1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Serpinh1 ELISA Kit (Rat) (OKEH06315) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinh1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 98%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Serpin H1
Protein Length
417
NCBI Gene Symbol
Serpinh1
Protein Name
P29457
Gene Name URL
Serpinh1
Nucleotide Accession Number
NM_017173.1
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