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ANXA3 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Annexin A3

Gene Aliases

35-alpha calcimedin; annexin A3; Annexin III; Annexin III (Lipocortin III) ; annexin-3; Anx3; LC3; lipocortin III; LRRGT00047; PAP-III; placental anticoagulant protein III.

Gene ID

25291

Accession Number

NP_036955.2

Reactivity

Rat, Rattus norvegicus

Target

Annexin A3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Anxa3 ELISA Kit (Rat) (OKEH06305) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Anxa3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Anxa3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Anxa3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 104%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Annexin A3

Protein Length

324

NCBI Gene Symbol

Anxa3

Protein Name

P14669

Gene Name URL

Anxa3

Nucleotide Accession Number

NM_012823.2

Frequently Asked Questions

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