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EGR1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Early growth response 1

Gene Aliases

Early growth response protein 1; EGR-1; Krox-24; nerve growth factor-induced protein A; Ngf1; Ngfi; NGFI-A; transcription factor Zif268; zif-268; zinc finger protein Krox-24.

Gene ID

24330

Accession Number

NP_036683.1

Reactivity

Rat, Rattus norvegicus

Target

Early growth response protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Egr1 ELISA Kit (Rat) (OKEH06297) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Egr1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Egr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Egr1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 102%

Sensitivity

39.3 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Early growth response protein 1

Protein Length

508

NCBI Gene Symbol

Egr1

Protein Name

P08154

Gene Name URL

Egr1

Nucleotide Accession Number

NM_012551.2

Frequently Asked Questions

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