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MIOX ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Myo-inositol oxygenase

Gene Aliases

Aldehyde reductase (aldose reductase) like 6; aldehyde reductase-like 6; Aldrl6; inositol oxygenase; kidney-specific protein 32; MI oxygenase; Myo-inositol oxygenase; renal-specific oxidoreductase; renal-specific oxido-reductase; RSOR.

Gene ID

252899

Accession Number

NP_665714.2

Reactivity

Rat, Rattus norvegicus

Target

Inositol oxygenase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Miox ELISA Kit (Rat) (OKEH06288) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Miox has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Miox is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Miox captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 85%

Sensitivity

0.17 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Inositol oxygenase

Protein Length

285

NCBI Gene Symbol

Miox

Protein Name

Q9QXN4

Gene Name URL

Miox

Nucleotide Accession Number

NM_145771.2

Frequently Asked Questions

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