LIFR ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
LIF receptor subunit alpha
Gene Aliases
Leukemia inhibitor factor receptor alpha-chain; leukemia inhibitory factor receptor; leukemia inhibitory factor receptor alpha; LIF receptor alpha; LIF-R.
Gene ID
81680
Accession Number
NP_112310.1
Reactivity
Rat, Rattus norvegicus
Target
Leukemia inhibitory factor receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lifr ELISA Kit (Rat) (OKEH06282) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lifr has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lifr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lifr captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 83%
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Leukemia inhibitory factor receptor
Protein Length
1093
NCBI Gene Symbol
Lifr
Protein Name
O70535
Gene Name URL
Lifr
CAS Number
7732-18-5
Nucleotide Accession Number
NM_031048.1
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