What is the catalog number for EPCAM ELISA Kit (Rat)?

The catalog number for EPCAM ELISA Kit (Rat) is OKEH06192-96W.

What sizes are available for EPCAM ELISA Kit (Rat)?

EPCAM ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is EPCAM ELISA Kit (Rat) in stock?

EPCAM ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

EPCAM ELISA Kit (Rat)

Selected Variant

OKEH06192-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Epithelial cell adhesion molecule

Gene Aliases

Egp314; ep-CAM; epithelial cell adhesion molecule; epithelial glycoprotein 314; Protein D5.7A; Tacstd1; tumor-associated calcium signal transducer 1.

Gene ID

171577

Accession Number

NP_612550.1

Reactivity

Rat, Rattus norvegicus

Target

Epithelial cell adhesion molecule

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Epcam ELISA Kit (Rat) (OKEH06192) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Epcam has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Epcam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Epcam captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 103%

Sensitivity

0.177 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Epithelial cell adhesion molecule

Protein Length

315

NCBI Gene Symbol

Epcam

Protein Name

O55159

Gene Name URL

Epcam

Nucleotide Accession Number

NM_138541.1

Price

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