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LITAF ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Lipopolysaccharide-induced TNF factor

Gene Aliases

Pig7, EET-1

Gene ID

65161

Accession Number

NP_001099205.1

Reactivity

Rat

Target

Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Litaf ELISA Kit (Rat) (OKEH06185) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Litaf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Litaf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Litaf captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156 - 10ng/ml

Recovery

Mean recovery when spiking into Serum and Plasma = 93%

Sensitivity

0.083 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

Protein Length

161

NCBI Gene Symbol

LITAF

Protein Name

Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

Gene Name URL

LITAF

Nucleotide Accession Number

NM_001105735.2

Frequently Asked Questions

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