LHCGR ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Luteinizing hormone/choriogonadotropin receptor
Gene Aliases
LH receptor; LH/CG receptor; LH/CG-R; Lhr; LSHR; LSH-R; luteinizing hormone receptor; luteinizing hormone/chorionic gonadotropin receptor; lutropin-choriogonadotropic hormone receptor.
Gene ID
25477
Accession Number
NP_037110.1
Reactivity
Rat, Rattus norvegicus
Target
Lutropin-choriogonadotropic hormone receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lhcgr ELISA Kit (Rat) (OKEH06173) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lhcgr has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lhcgr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lhcgr captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 86%
Sensitivity
0.091 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Lutropin-choriogonadotropic hormone receptor
Protein Length
700
NCBI Gene Symbol
Lhcgr
Protein Name
P16235
Gene Name URL
Lhcgr
Nucleotide Accession Number
NM_012978.1
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