LAMB2 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Laminin subunit beta 2
Gene Aliases
Laminin chain B3; laminin chain beta 2; laminin subunit beta-2; laminin, beta 2; laminin-11 subunit beta; laminin-14 subunit beta; laminin-15 subunit beta; laminin-3 subunit beta; laminin-4 subunit beta; laminin-7 subunit beta; laminin-9 subunit beta; SLAM; S-LAM beta; S-laminin subunit beta.
Gene ID
25473
Accession Number
NP_037106.1
Reactivity
Rat, Rattus norvegicus
Target
Laminin subunit beta-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Lamb2 ELISA Kit (Rat) (OKEH06161) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lamb2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lamb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lamb2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 105%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Laminin subunit beta-2
Protein Length
1801
NCBI Gene Symbol
Lamb2
Protein Name
P15800
Gene Name URL
Lamb2
CAS Number
7732-18-5
Nucleotide Accession Number
NM_012974.1
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