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FCGR3 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Fc fragment of IgG receptor IIa, low affinity immunoglobulin gamma Fc region receptor III-like

Gene Aliases

Fc fragment of IgG low affinity IIa receptor for (CD32) ; Fc fragment of IgG, low affinity IIa, receptor (CD32) ; Fc receptor, IgG, low affinity III; fc-gamma RIII; Fcgr3; Fcgr3a; fcRIII; igG Fc receptor III; low affinity immunoglobulin gamma Fc region receptor III.

Gene ID

100911825, 116591

Accession Number

NP_001129464.1

Reactivity

Rat, Rattus norvegicus

Target

Low affinity immunoglobulin gamma Fc region receptor III

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Fcgr3 ELISA Kit (Rat) (OKEH06138) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fcgr3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Fcgr3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fcgr3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 95%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Low affinity immunoglobulin gamma Fc region receptor III

Protein Length

267

NCBI Gene Symbol

Fcgr2a, LOC100911825

Protein Name

P27645

Gene Name URL

Fcgr2a, LOC100911825

Nucleotide Accession Number

NM_001135992.1

Frequently Asked Questions

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