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CSF1R ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Macrophage colony-stimulating factor 1 receptor

Gene Aliases

CSF-1 receptor; Proto-oncogene c-Fms.

Gene ID

307403

Accession Number

NP_001025072.1

Reactivity

Rat, Rattus norvegicus

Target

Macrophage colony-stimulating factor 1 receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Csf1r ELISA Kit (Rat) (OKEH06135) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Csf1r has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Csf1r is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Csf1r captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 87%

Sensitivity

0.312 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Macrophage colony-stimulating factor 1 receptor

Protein Length

978

NCBI Gene Symbol

CSF1R

Protein Name

Q00495

Gene Name URL

CSF1R

Nucleotide Accession Number

NM_001029901.1

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