Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

PRAP1 ELISA Kit (Rat)

Selected Variant

OKEH06130-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Proline-rich acidic protein 1

Gene Aliases

Proline-rich acidic protein 1; Upa; uterine-specific proline-rich acidic protein.

Gene ID

60574

Accession Number

NP_113857.1

Reactivity

Rat, Rattus norvegicus

Target

Proline-rich acidic protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Prap1 ELISA Kit (Rat) (OKEH06130) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prap1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Prap1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prap1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 97%

Sensitivity

0.409 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Proline-rich acidic protein 1

Protein Length

152

NCBI Gene Symbol

Prap1

Protein Name

Q9ES75

Gene Name URL

Prap1

Nucleotide Accession Number

NM_031669.1

Price

Sign In for Pricing

Frequently Asked Questions