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TSC22D1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

TSC22 domain family, member 1

Gene Aliases

Regulatory protein TSC-22; Tgfb1i4; TGFB-stimulated clone 22 homolog; transforming growth factor beta 1 induced transcript 4; Transforming growth factor beta stimulated clone 22; transforming growth factor beta-1-induced transcript 4 protein; TS22A; TSC-22; TSC22 domain family protein 1.

Gene ID

498545

Accession Number

NP_037175.1

Reactivity

Rat, Rattus norvegicus

Target

TSC22 domain family protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Tsc22d1 ELISA Kit (Rat) (OKEH06128) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tsc22d1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tsc22d1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tsc22d1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 88%

Sensitivity

42 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat TSC22 domain family protein 1

Protein Length

143

NCBI Gene Symbol

Tsc22d1

Protein Name

P62501

Gene Name URL

Tsc22d1

Nucleotide Accession Number

NM_013043.2

Frequently Asked Questions

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