TSC22D1 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
TSC22 domain family, member 1
Gene Aliases
Regulatory protein TSC-22; Tgfb1i4; TGFB-stimulated clone 22 homolog; transforming growth factor beta 1 induced transcript 4; Transforming growth factor beta stimulated clone 22; transforming growth factor beta-1-induced transcript 4 protein; TS22A; TSC-22; TSC22 domain family protein 1.
Gene ID
498545
Accession Number
NP_037175.1
Reactivity
Rat, Rattus norvegicus
Target
TSC22 domain family protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tsc22d1 ELISA Kit (Rat) (OKEH06128) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tsc22d1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Tsc22d1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tsc22d1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88%
Sensitivity
42 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat TSC22 domain family protein 1
Protein Length
143
NCBI Gene Symbol
Tsc22d1
Protein Name
P62501
Gene Name URL
Tsc22d1
Nucleotide Accession Number
NM_013043.2
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