What is the catalog number for PPP1R1A ELISA Kit (Rat)?

The catalog number for PPP1R1A ELISA Kit (Rat) is OKEH06126-96W.

What sizes are available for PPP1R1A ELISA Kit (Rat)?

PPP1R1A ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is PPP1R1A ELISA Kit (Rat) in stock?

PPP1R1A ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

PPP1R1A ELISA Kit (Rat)

Selected Variant

OKEH06126-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Protein phosphatase 1, regulatory (inhibitor) subunit 1A

Gene Aliases

I-1; IPP-1; PP1 inhibitor 1; protein phosphatase 1 regulatory subunit 1A; protein phosphatase inhibitor 1.

Gene ID

58977

Accession Number

NP_073167.1

Reactivity

Rat, Rattus norvegicus

Target

Protein phosphatase 1 regulatory subunit 1A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Ppp1r1a ELISA Kit (Rat) (OKEH06126) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppp1r1a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ppp1r1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppp1r1a captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 93%

Sensitivity

42.1 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Protein phosphatase 1 regulatory subunit 1A

Protein Length

171

NCBI Gene Symbol

Ppp1r1a

Protein Name

P19103

Gene Name URL

Ppp1r1a

Nucleotide Accession Number

NM_022676.3

Price

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