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CSNK2B ELISA Kit (Rat)

Selected Variant

OKEH06122-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Casein kinase 2 beta

Gene Aliases

Casein kinase 2, beta polypeptide; casein kinase 2, beta subunit; casein kinase II beta subunit; casein kinase II subunit beta; CK II beta; Ck2; Csnk2b-Ly6g5b; phosvitin.

Gene ID

81650

Accession Number

NP_001030315.1

Reactivity

Rat, Rattus norvegicus

Target

Casein kinase II subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Csnk2b ELISA Kit (Rat) (OKEH06122) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Csnk2b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Csnk2b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Csnk2b captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 98%

Sensitivity

15.8 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Casein kinase II subunit beta

Protein Length

215

NCBI Gene Symbol

Csnk2b

Protein Name

P67874

Gene Name URL

Csnk2b

Nucleotide Accession Number

NM_001035238.2

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