PRDX6 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Peroxiredoxin 6
Gene Aliases
1-Cys peroxiredoxin;1-Cys PRX; acidic calcium-independent phospholipase A2; aiPLA2; antioxidant protein 2; glutathione-dependent peroxiredoxin; LPC acyltransferase 5; LPCAT-5; lyso-PC acyltransferase 5; lysophosphatidylcholine acyltransferase 5; non-selenium glutathione peroxidase; NSGPx; peroxiredoxin-6; thiol-specific antioxidant protein.
Gene ID
94167
Accession Number
NP_446028.1
Reactivity
Rat, Rattus norvegicus
Target
Peroxiredoxin-6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Prdx6 ELISA Kit (Rat) (OKEH06109) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prdx6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Prdx6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prdx6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 106%
Sensitivity
31.27 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Peroxiredoxin-6
Protein Length
224
NCBI Gene Symbol
Prdx6
Protein Name
O35244
Gene Name URL
Prdx6
Nucleotide Accession Number
NM_053576.2
Frequently Asked Questions
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