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PRDX6 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Peroxiredoxin 6

Gene Aliases

1-Cys peroxiredoxin;1-Cys PRX; acidic calcium-independent phospholipase A2; aiPLA2; antioxidant protein 2; glutathione-dependent peroxiredoxin; LPC acyltransferase 5; LPCAT-5; lyso-PC acyltransferase 5; lysophosphatidylcholine acyltransferase 5; non-selenium glutathione peroxidase; NSGPx; peroxiredoxin-6; thiol-specific antioxidant protein.

Gene ID

94167

Accession Number

NP_446028.1

Reactivity

Rat, Rattus norvegicus

Target

Peroxiredoxin-6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Prdx6 ELISA Kit (Rat) (OKEH06109) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prdx6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Prdx6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prdx6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 106%

Sensitivity

31.27 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Peroxiredoxin-6

Protein Length

224

NCBI Gene Symbol

Prdx6

Protein Name

O35244

Gene Name URL

Prdx6

Nucleotide Accession Number

NM_053576.2

Frequently Asked Questions

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