CHRM3 ELISA Kit (Rat)
Selected Variant
OKEH06097-96W
Size: 96 Well
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Cholinergic receptor, muscarinic 3
Gene Aliases
Cholinergic receptor, muscarinic 3, cardiac; muscarinic acetylcholine receptor M3.
Gene ID
24260
Accession Number
NP_036659.2
Reactivity
Rat, Rattus norvegicus
Target
Muscarinic acetylcholine receptor M3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Chrm3 ELISA Kit (Rat) (OKEH06097) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Chrm3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Chrm3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Chrm3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 82%
Sensitivity
0.18 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Muscarinic acetylcholine receptor M3
Protein Length
589
NCBI Gene Symbol
Chrm3
Protein Name
P08483
Gene Name URL
Chrm3
Nucleotide Accession Number
NM_012527.2
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