What is the catalog number for PXN ELISA Kit (Rat)?

The catalog number for PXN ELISA Kit (Rat) is OKEH06095-96W.

What sizes are available for PXN ELISA Kit (Rat)?

PXN ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is PXN ELISA Kit (Rat) in stock?

PXN ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

PXN ELISA Kit (Rat)

Selected Variant

OKEH06095-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Paxillin

Gene Aliases

Inverted formin-2-like; myocardial ischemic preconditioning associated protein 7; paxillin.

Gene ID

360820

Accession Number

NP_001012147.1

Reactivity

Rat, Rattus norvegicus

Target

Paxillin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Pxn ELISA Kit (Rat) (OKEH06095) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pxn has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pxn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pxn captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 101%

Sensitivity

31.29 pg/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Paxillin

Protein Length

586

NCBI Gene Symbol

Pxn

Protein Name

Q66H76

Gene Name URL

Pxn

Nucleotide Accession Number

NM_001012147.1

Price

Frequently Asked Questions

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