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ENTPD5 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

Ectonucleoside triphosphate diphosphohydrolase 5

Gene Aliases

Ectonucleoside triphosphate diphosphohydrolase 5; GDPase ENTPD5; guanosine-diphosphatase ENTPD5; NTPDase 5; Pcph; UDPase ENTPD5; uridine-diphosphatase ENTPD5.

Gene ID

314312

Accession Number

NP_955426.1

Reactivity

Rat, Rattus norvegicus

Target

Ectonucleoside triphosphate diphosphohydrolase 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Entpd5 ELISA Kit (Rat) (OKEH06077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Entpd5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Entpd5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Entpd5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 98%

Sensitivity

0.092 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Rat Ectonucleoside triphosphate diphosphohydrolase 5

Protein Length

427

NCBI Gene Symbol

Entpd5

Protein Name

Q6P6S9

Gene Name URL

Entpd5

Nucleotide Accession Number

NM_199394.1

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