DDOST ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
Gene Aliases
DDOST 48 kDa subunit; dolichyl-diphosphooligosaccharide-protein glycosyltransferase; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) ; dolichyl-di-phosphooligosaccharide-protein glycotransferase; oligosaccharyl transferase 48 kDa subunit.
Gene ID
313648
Accession Number
NP_001012104.1
Reactivity
Rat, Rattus norvegicus
Target
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ddost ELISA Kit (Rat) (OKEH06058) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ddost has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ddost is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ddost captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 96%
Sensitivity
0.159 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Protein Length
441
NCBI Gene Symbol
Ddost
Protein Name
Q641Y0
Gene Name URL
Ddost
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001012104.1
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