KCNB2 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
Gene Name
Potassium voltage-gated channel subfamily B member 2
Gene Aliases
CDRK; Voltage-gated potassium channel subunit Kv2.2.
Gene ID
117105
Accession Number
NP_446452.2
Reactivity
Rat, Rattus norvegicus
Target
Potassium voltage-gated channel subfamily B member 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Kcnb2 ELISA Kit (Rat) (OKEH06020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kcnb2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Kcnb2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kcnb2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 101%
Sensitivity
0.084 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Potassium voltage-gated channel subfamily B member 2
Protein Length
907
NCBI Gene Symbol
KCNB2
Protein Name
Q63099
Gene Name URL
KCNB2
CAS Number
7732-18-5
Nucleotide Accession Number
NM_054000.2
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